ارزیابی کمی پروتئینوری با استفاده از نسبت پروتئین به کراتینین در نمونه‌های تصادفی ادرار افراد مبتلا به بیماریهای کلیوی

Quantitation of urinary protein excretion is important for diagnostic and prognostic purposes and to achieve the best treatment. The method most commonly used to measure urinary protein in 24 hour urine samples, are time consuming and often inaccurate. This study performed on 101 persons. Urine samples were collected three times aday. The protein/creatinine ratio(mg:mg) was measured and compared to the 24 hour urine samples of 20 healthy persons and 81 patients with a wide spectrum of renal function and proteinuria. We used pesce and strand method to measure protein urine samples. In addition, we used test tape for determination of protein in urine samples. Our findings revealed a close correlation between the amount of protein in random samples and 24 hour urine excretion. The higher correlation was found in urine samples, collected at 11.00 A.M and the lowest correlation in samples obtained at 6.00 A.M. There are higher correlation in patients who 24 hour urinary protein excretion are less than 1g. By taking in to consideration the effect of different rates of creatinine excretion, we conclude that determination of the protein/creatinine ratio in single urine samples obtained during normal day light activity can replace the 24 hour urine collection in the clinical quantitation of proteinuria. However, this method is simple, fast and reliable compared to the test tape method which gave false positive and false negative results.